TABLE 3.
Kinetic parameters of NAD-ME2, -H, and -1q in the presence of 50 μm CoA
The indicated values are the average of at least three different experiment with no more than 5% S.D.
| 50 μm CoA |
||||
|---|---|---|---|---|
| pH | Kml-malate | kcat | kcat/Km malate | |
| mm | s−1 | |||
| NAD-ME2 | 6.8 | 0.20 | 69.1 | 345 |
| NAD-MEH | 6.8 | 0.80a | 40.6 | 51 |
| NAD-ME1q | 6.8 | 0.06 | 45.0 | 750 |
a S0.5; nH = 1.3.