FIGURE 7.

miR-9 inhibits hypertrophic responses in the cellular model. A, miR-9 inhibits myocardin expression in cells treated with Iso. Cardiomyocytes were infected with the adenoviral miR-9 or the mutated miR-9 (miR-9-mut) at a moi of 100. 24 h after infection, the cells were treated with 10 μm Iso. Myocardin expression was analyzed by immunoblot. B, miR-9 inhibits myocardin expression in cells treated with Aldo. Cardiomyocytes were treated as described for A, except that 1 μm Aldo was used. Myocardin expression was analyzed by immunoblot. C–F, miR-9 attenuates hypertrophic responses induced by Iso. Cardiomyocytes were treated as described for A. C, sarcomere organization. Bar, 20 μm. D, cell surface area measurement. E, protein/DNA ratio. F, β-MHC expression levels. *, p < 0.05 versus Iso alone. The data are expressed as the means ± S.E. of three independent experiments.