FIGURE 5.

Smc6 prevents the accumulation of X-structures during senescence. A, representative two-dimensional gel analysis of replication intermediates accumulated in tlc1Δ and tlc1Δ smc6-9 strains at the telomere visualized with a Y′-Long-specific probe. I and T refer to X-spikes coming from internal and terminal replicating telomere fragments, respectively; H refers to an apparent hybrid X-spike. B–D, comparisons of the ratio of X-shaped molecules to the replication arc within the telomere, near ARS304 and the rDNA locus for tlc1Δ and tlc1Δ smc6-9 cells at 45 PD after spore germination. The means ± S.E. (error bars) from three independent samples/genotype are shown. The respective p values are p < 0.0001, p = 0.15, and p = 0.38.