TABLE 2.
Species specificity of PrPSc-specific mAbs
Reactivity was graded as (−, +, or ++) in accordance with the results of at least three immunoprecipitation assays. In the case of mouse-adapted bovine spongiform encephalopathy (BSE), PrPSc strongly bound to control IgG, and its reactivity was determined by comparison with the intensity of the control IgG (same as control, ±; stronger than control, +). CWD, chronic wasting disease.
| mAb | Scrapie |
CWD, deer | BSE |
Subclass | |||
|---|---|---|---|---|---|---|---|
| Sheep | Mouse | Hamster | Cattle | Mouse | |||
| 3B7 | − | ++ | ++ | ++ | − | + | IgG2a |
| 2C4 | − | ++ | ++ | + | − | ± | IgG2b |
| 1B12 | − | ++ | ++ | + | − | ± | IgG2b |
| 3H6 | − | ++ | − | − | − | ± | IgG2b |