FIGURE 2.

hynac5 is expressed at the base of the tentacles. Whole mount in situ hybridization reveals strong expression of hynac5 transcripts at the tentacle base in adult animals (A) and buds (D and G). By comparison hynac2 and hynac3 transcripts are uniformly distributed (B and C). During bud formation, hynac5 transcripts appear as soon as tentacles begin to appear (G) similar to hynac2 and hynac3 (E and F, and H and I). Expression of hynac5 is strongest at the upper side (oral) of the tentacles (A); primary magnifications are 4× (A–C) and 20× (d–I).