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. 2010 Feb 16;285(16):11958–11965. doi: 10.1074/jbc.M109.059998

FIGURE 6.

FIGURE 6.

Ion selectivity of HyNaC2/3/5. A, the HyNaC5 subunit does not change reversal potentials. Channels were activated with 30 μm (HyNaC2/3) or 0.3 μm Hydra-RFamide I (HyNaC2/3/5), respectively, and reversal potentials measured by stepping to the indicated holding potentials for 3 s. B, the HyNaC5 subunit decreases currents carried by K+. Left, representative current traces of whole oocytes either expressing HyNaC2 and -3 or HyNaC2, -3, and -5. Channels were activated in the presence of different monovalent cations. Right, bar graphs illustrating the amplitude of the Li+ and K+ current relative to the Na+ current; black bars represent HyNaC2/3/5, white bars HyNaC2/3. Error bars represent S.E. (12 oocytes); HyNaCs were activated with 30 μm (HyNaC2/3) or 0.3 μm Hydra-RFamide I (HyNaC2/3/5), respectively. In the presence of HyNaC5, amplitudes of Li+ and K+ currents were significantly reduced relative to the amplitude of the Na+ current. **, p < 0.01; ***, p < 0.001, two-tailed t test.