FIGURE 8.

Amiloride delays the glutathione induced feeding response. H. magnipapillata were relaxed in plain medium or medium containing 100 μm amiloride. At time 0, glutathione was added to a final concentration of 10 μm. Every 30 s, the number of animals moving their tentacles was recorded. A statistically significant delay in the response to glutathione (GSH) was detected in the presence of amiloride. The calculated p value is < 0.05 as indicated (by an asterisk), whereas it is 0.06 for the time point 1:00.