FIGURE 1.

Isolation of AF9-binding proteins. THP-1 whole cell extract was pre-adsorbed to a FLAG peptide affinity matrix and was then applied to a FLAG-AF9-ct column (amino acids 475–568 of AF9). Bound proteins were eluted with 1 m NaCl and 100 mm glycine. Eluted proteins were separated by SDS-PAGE and stained with Coomassie Brilliant Blue. Bands 1–6 were excised from the gel and identified by mass spectroscopy. The 85-kDa band is the β isoform of Hsp90. The gel shown is the original and only replicate.