FIGURE 4.

Effect of LXRα siRNA in cAMP- or 22-hydroxycholesterol-induced up-regulation of renin expression in human MSCs. Human MSCs were transfected with LXRα siRNA or control siRNA. 1 mmol/liter 8-bromo-cyclic AMP (cAMP) (A) or 0.1 μmol/liter LXRα (B) natural ligand 22-hydroxycholesterol (22OH-C) was added to media after 16 h of serum depletion. Cells were harvested after 6 h of pharmacological treatment, and total RNA was analyzed by quantitative RT-PCR for mRNA expression of LXRα and Renin. Data are -fold changes versus the control siRNA group. Data are the mean ± S.E.; n = 5 in each group; *, p < 0.05 versus control siRNA group.