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. 2010 Jan 29;285(16):11974–11982. doi: 10.1074/jbc.M109.099671

FIGURE 5.

FIGURE 5.

Effect of cAMP on the expression of Ren1c gene in mMSC/GFP and mMSC/LXRα/GFP. One mmol/liter 8-bromo-cyclic AMP (cAMP) was added to media after 16 h of serum depletion. Murine MSCs overexpressing GFP (mMSC/GFP) or GFP-LXRα (mMSC/LXRα/GFP) with or without cAMP treatment were harvested after 6 h of pharmacological treatment, and total RNA was analyzed by quantitative RT-PCR for mRNA expression of Ren1c. Without Tx, without the treatment group. Data are -fold changes versus mMSC/GFP without Tx. Data are the mean ± S.E.; n = 3. *, p < 0.05 versus mMSC/GFP without Tx. #, p < 0.05 versus mMSC/GFP, cAMP. +, p < 0.05 versus mMSC/LXRα/GFP without Tx.