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. 2010 Jan 29;285(16):11974–11982. doi: 10.1074/jbc.M109.099671

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Phenotypic changes of mMSC/LXRα/GFP induced by the prolonged treatment with cAMP or 22-hydroxycholesterol. Prolonged treatment of mMSC/LXRα/GFP with cAMP (A, B, and E) or 22-hydroxycholesterol (C and D) was performed. The arrows show granules in cytoplasm (A, cAMP treatment for 3 weeks; C, 22-hydroxycholesterol treatment for 6 weeks). Immunocytochemistry for renin antibody exhibited that granules were positive for renin (B, cAMP treatment for 2 weeks; D, 22-hydroxycholesterol treatment for 6 weeks). E, negative control for renin staining using isotype control immunoglobulin G. Original magnification, ×400.