TABLE 1.
Primers used in this study
Lowercase type in primers 1242 and 1243 indicates an additional sequence present in the given primers. This sequence is the recognition site of restriction enzyme SalI. Underlined letters in primers 1244, 1245, 1246, and 1247 indicate the changed nucleotides compared with hSP-A cDNA sequences.
| Primer | Sequence (5′ to 3′) | Comments |
|---|---|---|
| 1458 | GGAGGGCCAGGAACAAACAGG | Human SP-C promoter, sense |
| 1459 | CCAGGAGTGCCGGGGATACCAG | Human SP-A exon I, antisense |
| 1433 | CACATATAAGACCCTGGTCACACCT | Human SP-C promoter, sense |
| 292 | CCATTATTCCCAGGAGGACTAGGTG | Human SP-A2 antisense at exon II |
| 293 | CCATCATTTCCAGGAGGACTAGGCA | Human SP-A1 antisense at exon II |
| 68 | CAGGTCGACTGCCACAGAGACCTCAGAGT | Human SP-A antisense at 3′-UTR |
| 1549 | CCTGTCTGGTAGCAAGTAGAGCT | Mouse SP-A sense |
| 1542 | CCAGTGACATCGGGAGGAAATCC | Mouse SP-A antisense |
| 1280 | GTGGGGTGGGATTAGATAAATGC | Mouse SP-A for SP-A (−/−) KO mouse |
| 1242 | GGCCCGGGgtcgacTGGAGGCTCTGTGTGTGGG | Human SP-A, sense with SalI site |
| 1243 | GGCCCGGGgtcgacTGCCACAGAGACCTCAGAGT | Human SP-A, antisense with SalI site |
| 1244 | ATGTGCCAGAGCAGGAGGCCGCATTGCTGTC | Human SP-A, sense with a mutated nucleotide |
| 1245 | GACAGCAATGCGGCCTCCTGCTCTGGCACAT | Human SP-A, antisense with a mutated nucleotide |
| 1246 | GTGAAGAAGTACAACACCTATGCCTATGTAGGC | Human SP-A, sense with a mutated nucleotide |
| 1247 | GCCTACATAGGCATAGGTGTTGTACTTCTTCAC | Human SP-A, antisense with a mutated nucleotide |
| 1634 | CCTTGCCCCGTGCCTATGAG | Human SP-C 5′-flanking region, antisense |
| 1636 | TGAGCAACTCTGGCCAAGCATAA | Human SP-A 3′-UTR, sense |