FIGURE 6.

USP17 blunts Ras ERK and JNK MAPK signaling. A, HeLa cells were transfected with N-Ras along with control (empty vector), USP17, and USP17CS. Cells were serum-starved then stimulated with EGF (100 ng/ml) for the indicated times. Cell lysates were immunoblotted with pERK, pJNK, Ras, and USP17 antibodies. pERK and pJNK blots were reprobed for total ERK and total JNK, respectively. Densitometry of p44 pERK1 (B) and pJNK (C). Statistics compares 0 and 30 min of EGF treatment. *** indicates p < 0.001, and ns is not significant.