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. 2010 Feb 22;285(16):12037–12046. doi: 10.1074/jbc.M109.064808

FIGURE 6.

FIGURE 6.

siRNA knockdown of claudin-2 reduces IL-13-mediated barrier dysfunction. A, claudin-2 protein content was reduced by 71 ± 7 and 58 ± 1% in the absence and presence of IL-13 (2 ng/ml), respectively, by claudin-2-targeted siRNA (relative to control siRNA). B, claudin-2-targeted siRNA prevented IL-13-induced increases in tight junction-associated claudin-2. Bar, 10 μm. C, claudin-2-targeted siRNA (all hatched bars) blocked IL-13-induced (light gray bars) increases in PNa+/PCl−. D, siRNA-mediated claudin-2 knockdown (all hatched bars) inhibited only a small fraction of IL-13-induced (light gray bars) TER loss (**, p ≤ 0.01, S.E.).