FIGURE 8.

Claudin-2 (green) and IL-13 expression are increased in CA-MLCK mice. A, mucosal IL-13 mRNA transcripts are increased in CA-MLCK transgenic mice (gray bars) relative to wild type littermates (white bars). B, claudin-2 expression in colonocytes from CA-MLCK transgenic mice was markedly increased compared with wild type (WT) littermates. C, claudin-2 (green) expression was limited to crypt epithelium in wild type mice but was also present in surface colonocytes of CA-MLCK transgenic mice (arrow). Bar, 40 μm. F-actin (red) and nuclei (blue) are shown for orientation. D, claudin-2 staining was increased in surface colonocytes in biopsies from patients with ulcerative colitis relative to colon biopsies from healthy subjects. Bar, 40 μm (*, p ≤ 0.05, S.E.).