FIGURE 3.

The full-length Hv1 is mainly expressed in intracellular sites in HeLa cells, but truncation of the C or N terminus alone alters localization. HeLa cells grown on glass coverslips were transfected with the expression plasmids pHv1-EGFP, pΔC-Hv1-EGFP, and pΔN-Hv1-EGFP and observed by an FV1000S-IX81 focal microscope. The localizations of with full-length (A–C), C terminus-deleted (D–F), and N terminus-deleted (G–I) Hv1 are shown in green. B, E, and H, the phase-contrast images of the same fields corresponding to A, D, and G, respectively. C, F, and I, images that merge A and B, D and E, and G and H, respectively.