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Activation of AtSnRK1 catalytic subunits by AtSnAK. AtSnRK1.1-GST and AtSnRK1.2-GST (1 μg of each preparation) were incubated with each AtSnAK-GST (0.5 μg of each preparation) in the reconstituted assay containing 2 μCi of [γ-32P]ATP, 30 μm ATP, and 90 μm AMARA peptide, at 30 °C for 30 min. Radioactivity incorporated onto the AMARA peptide was counted. The results are the average of three independent experiments ± S.D. (error bars).
