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The role mutation plays in nano-cage protein thermal stability and ability to refold after denaturation. Top, CD spectra of the BFR derivatives before denaturation (solid lines) and after slow cooling post-thermal denaturation (dashed lines). Bottom, thermal transitions of the BFR derivatives as monitored by CD. Schematics follow the convention in Fig. 1, and the coloring follows that of Fig. 3. deg, degrees.
