Cytokine stimulation by C. albicansMNT1 null mutants. PBMCs were coincubated with either heat-killed (A) or live (B) yeast cells in a ratio monocyte: yeast 5: 1 for 24 h, and the cytokine concentrations were determined. The strains were: NGY152 (wild type, WT), NGY1227 (Camnt3-Camnt5Δ), NGY1228 (Camnt3-Camnt5Δ + CaMNT3), NGY1229 (Camnt3-Camnt5Δ + CaMNT5), NGY516 (Camnt4-Camnt5Δ), NGY517 (Camnt4-Camnt5Δ + CaMNT4), NGY518 (Camnt4-Camnt5Δ + CaMNT5), NGY527 (Camnt3-Camnt4-Camnt5Δ), NGY528 (Camnt3-Camnt4-Camnt5Δ + CaMNT3), NGY529 (Camnt3-Camnt4-Camnt5Δ + CaMNT4), and NGY530 (Camnt3-Camnt4-Camnt5Δ + CaMNT5). The data represent the means ± S.D. *, p < 0.05.