TABLE 1.

Binding kinetics of monomeric CTLD toward hTNFα and inhibitory constant of the trimeric CTLD

Maturation round	Clone	KDa	kon	koff	Ki	95% confidence intervals
		m	m−1s−1	s−1	ng/ml
Primary	TN2	>5.0 × 10−5	NDb	NDb	NDb
First	TN2-B	7.3 × 10−8	1.3 × 107	9.2 × 10−1	>10 × 106
Second	TN2-B1	1.2 × 10−8	2.5 × 105	2.9 × 10−3	1440	1026–2017
Third	TN2-B1-C31	3.4 × 10−10	2.6 × 106	8.9 × 10−4	2.8	1.6–6.6

^a Primary, first, and second clones were analyzed with ∼500 RU ligand immobilized, and the third clone was analyzed with ∼50 RU. T-values > 100. RU designates Biacore response unit; T-value is defined as parameter value divided by S.E.

^b ND, not detectable.