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The Nss mutant has a defect in Fap1 glycosylation. Whole cell extracts prepared from the same number of bacterial cells (1 × 108) of wild type (WT), fap1 mutant (fap1−), nss mutant (nss−), nss mutant transformed with pVPT-CHSV (nss−/pVPT), and nss mutant transformed with pVPT-Nss containing the full-length nss gene (nss−/pVPT-nss) were subjected to Western blotting analysis using Fap1-specific mAbs E42, D10, and F51.
