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Nss enzymatic activity determined by an in vitro glycosylation assay. A, Nss is a glucosyltransferase determined by an in vitro glycosyltransferase assay. B, UDP-glucose effectively inhibited the transfer of 3H-labeled UDP-glucose to the GlcNAc-modified Fap1 polypeptide. C, [3H]UDP-GlcNAc is not an active sugar donor for Nss. HI, heat inactivated.
