FIGURE 2.

Identification of a linear pro-NT binding site within sortilin. A and B, sortilin represented as 273 overlapping peptides generated by SPOT synthesis on a cellulose membrane with peptides 64–69 binding to HisS-BDNFpro, as detected by either HRP-S-protein (A, SPOT numbers 67–69) or antibody immunoassay against the polyhistidine tag (B, SPOT numbers 64–66). C, peptide sequences of the six peptides 64–69 found to interact with the pro-domains of BDNF and NGF. Residues corresponding to the sortilin fragment 163–174 are boxed. D, SPR analysis of the 12-mer sortilin-derived peptide (residues ¹⁶³RIFRSSDFAKNF¹⁷⁴) binding to flow cells with immobilized HisS-BDNFpro and HisS-NGFpro.