FIGURE 3.

Substitution analysis of the linear sortilin sequence. A, a representative substitutional binding analysis of HisS-BDNFpro to peptides with the wild-type sortilin sequence ¹⁶³RIFRSSDFAKNFVQTD¹⁷⁸ listed to the left on the membrane and detection using the anti-histidine immunoassay. B, binding assays as demonstrated in panel A were carried out for both HisS-BDNFpro and HisS-NGFpro, and signal intensities, measured in Boehringer Light Units (BLU), were quantified for each peptide. The percentage of replacement variability (V) of each sequence position have been calculated (V = BLU/20 × 100%) and plotted against each amino acid within the peptide (residues 163–178).