FIGURE 5.

Differential binding of pro-BDNF and neurotension to sortilin and pro-sortilin. A, SPR analysis showing that unprocessed pro-BDNF (50 nm) binds nearly as efficiently to the receptor in the presence (pro-sortilin) as in the absence (sortilin) of the receptor propeptide. Pro-sortilin is a receptor variant unable to cleave off the propeptide due to mutation of the furin recognition site. B, binding of neurotensin (20 μm) is strongly ablated for pro-sortilin as compared with binding to sortilin without the presence of its propeptide.