TABLE 1.
Alanine variant | KD ± S.D. | KDmut/wt | Charge variant | KD ± S.D. | KDmut/wt |
---|---|---|---|---|---|
nm | nm | ||||
M7A | 166 ± 15 | 2.4 | M7D | 382 ± 40 | 5.6 |
R11A | 264 ± 20 | 3.9 | R11D | 1063 ± 152 | 15.6 |
I14A | 203 ± 12 | 3.0 | I14D | u.m | u.m |
D18A | 37 ± 5 | 0.5 | D18K | 57 ± 3 | 0.8 |
E100A | 137 ± 12 | 2.0 | E100R | 230 ± 13 | 3.4 |
E109A | 95 ± 15 | 1.4 | E109R | 107 ± 16 | 1.6 |
S113A | 59 ± 3 | 0.9 | S113D | 396 ± 54 | 5.8 |
Q116A | u.m | u.m | Q116D | u.m | u.m |
K117A | 30 ± 4 | 0.4 | K117D | 1091 ± 147 | 16.0 |
I119A | 187 ± 32 | 2.8 | I119D | u.m | u.m |
H120A | 142 ± 23 | 2.1 | H120D | 2510 ± 480 | 36.9 |
L123A | 561 ± 52 | 8.3 | L123D | 3100 ± 236 | 45.6 |
WT | 68 ± 2 | 1.0 |