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. 2010 Feb 17;285(16):12232–12240. doi: 10.1074/jbc.M109.065920

FIGURE 2.

FIGURE 2.

Identification of the LZAP-binding regions of NLBP. A, diagram of wild type (WT) NLBP and serial deletion mutants (D1–D6). B and C, 293T cells were transfected with plasmids encoding Myc-LZAP and wild type SFB-NLBP or serial deletion mutants. Cell lysates were subjected to immunoprecipitation (IP) with anti-Myc (B) or anti-FLAG (C) antibodies and immunoblotted (W) with the antibodies on the left. The amounts of SFB triple-tagged NLBP and Myc-tagged LZAP in the lysates were analyzed by immunoblotting and shown in the bottom panels.