FIGURE 3.

Identification of the NLBP-binding region of LZAP. A, diagram of wild type (WT) LZAP and serial deletion mutants (D1–D4). B, 293T cells were transfected with plasmids encoding SFB-NLBP and wild type Myc-LZAP or serial deletion mutants. Cell lysates were subjected to immunoprecipitation (IP) with anti-FLAG antibody and immunoblotted (W) with the antibodies indicated on the left. Myc-tagged LZAP in the lysates were analyzed by immunoblotting and shown in the bottom panel. Mock, mock-transfected.