FIGURE 3.

IOI-42 promotes TRAIL-induced apoptosis of human prostate cancer cells. LNCaP cells were pretreated with IOI-42 (10 μm) for 4 h and then treated with TRAIL (200 ng/ml) for 48 h. Cells were stained with Annexin V/PI staining and followed by FACS analysis. A, the representative dot plot of apoptosis assay. Data presented the percentage of Annexin V-positive cells. B, data show means ± S.D. of triplicates from one experiment representative of three experiments. *, p < 0.05. C, cell lysates from A were immunoblotted by anti-caspase-8 p20 antibody. DMSO, dimethyl sulfoxide; PBS, phosphate-buffered saline. tBID, truncated BID.