TABLE 1.
Lipid binding properties of the ACCH domain
| Lipid | Kd | Changea |
|---|---|---|
| nm | -fold | |
| PM mimetic + PI(4)P (PC/PE/PS/PI/cholesterol/PI(4)P), 9:35:22:9:22:3) | 4.4 ± 1 | 1 |
| POPC/POPE/PI(3)P (75:20:5) | 18 ± 4 | 4 |
| POPC/POPE/PI(4)P (75:20:5) | 16 ± 3 | 4 |
| PM mimetic (PC/PE/PS/PI/cholesterol, 12:35:22:9:22) | 22 ± 4 | 5 |
| POPC/POPE/PI (60:20:20) | 95 ± 8 | 22 |
| POPC/POPE/PI(5)P (75:20:5) | 120 ± 10 | 27 |
| POPC/POPE/PI(3,4)P2 (75:20:5) | 300 ± 40 | 68 |
| POPC/POPE/PI(3,5)P2 (75:20:5) | 360 ± 50 | 82 |
| POPC/POPE/POPS (60:20:20) | 540 ± 40 | 120 |
| POPC/POPE/POPAb (60:20:20) | 610 ± 60 | 140 |
| POPC/POPE/PI(4,5)P2 (75:20:5) | NDc | |
| POPC/POPE/PI(3,4,5)P3 (75:20:5) | ND |
a Decrease of affinity relative to the Kd of the ACCH domain for plasma membrane + PI(4)P.
b1-Palmitoyl-2-oleoyl phosphatidic acid.
c ND, not determined.