Table 2.
Effects of treatments on PSC calcium responses evoked with maximal effective ATP concentration
| Treatments |
||||
|---|---|---|---|---|
| Chronic in vivoα-bungarotoxin blockade of nACHRs |
Chronic in vivo nerve stimulation |
|||
| PSC calcium response | Sham | Bungarotoxin | Control | Stimulated |
| Amplitude (%) | 189.1 ± 16.9 | 211.3 ± 17.7 | 726.4 ± 194.6 | 672.4 ± 100.1 |
| P= 0.370 | P= 0.823 | |||
| Duration (s) | 71.2 ± 7.2 | 102.7 ± 12.0 | 44.2 ± 6.3 | 47.6 ± 8.0 |
| P= 0.033* | P= 0.739 | |||
| Duration/amplitude (s/%) | 0.42 ± 0.04 | 0.6 ± 0.1 | 0.11 ± 0.02 | 0.08 ± 0.01 |
| P= 0.091 | P= 0.231 | |||
| Area under the curve (%/s2) | 8336.7 ± 1608.4 | 13866.6 ± 2734.4 | 23471.7 ± 10155.1 | 22531.3 ± 6716.1 |
| P= 0.096 | P= 0.943 | |||
| Half-width (s) | 34.7 ± 5.1 | 52.9 ± 8.1 | 23.0 ± 3.7 | 26.8 ± 5.6 |
| P= 0.071 | P= 0.555 | |||
| Delay (s) | 3.7 ± 0.3 | 3.4 ± 0.4 | 1.2 ± 0.4 | 1.2 ± 0.4 |
| P= 0.599 | P= 0.969 | |||
| Time-to-peak (s) | 12.3 ± 2.1 | 18.5 ± 3.6 | 6.7 ± 1.4 | 3.7 ± 0.3 |
| P= 0.631 | P= 0.082 | |||
| Rise slope (10–90%) (%/s) | 53.8 ± 9.6 | 57.9 ± 8.6 | 480.5 ± 168.0 | 356.5 ± 94.4 |
| P= 0.753 | P= 0.582 | |||
| Rise time (10–90%) (s) | 6.1 ± 1.2 | 6.5 ± 1.5 | 3.1 ± 1.2 | 1.5 ± 0.3 |
| P= 0.818 | P= 0.313 | |||
| Decay slope (10–90%) (%/s) | −4.7 ± 0.6 | −5.3 ± 0.6 | −29.5 ± 6.4 | −24.9 ± 3.6 |
| P= 0.464 | P= 0.551 | |||
| Decay time (10–90%) (s) | 41.5 ± 4.3 | 50.6 ± 9.5 | 24.2 ± 3.5 | 25.3 ± 4.3 |
| P= 0.381 | P= 0.847 | |||
Mean values and standard error for different PSC calcium response parameters measured in sham, α-BTx-treated, control and chronically stimulated NMJs. *Significant P values.