Table 3.

Effects of chronic nerve stimulation on PSC calcium responses evoked with submaximal effective ATP concentration

	Chronic in vivo nerve stimulation – ATP (10 μm)
	Highly sensitive PSCs		Moderately sensitive PSCs		Weakly sensitive PSCs
PSC calcium response	Control	Stimulated	Control	Stimulated	Control	Stimulated
Amplitude (%)	457.7 ± 68.1	364.5 ± 57.1	289.2 ± 52.7	305.8 ± 77.7	400.4 ± 80.9	522.5 ± 72.8
	P= 0.308		P= 0.872		P= 0.274
Duration (s)	25.2 ± 2.9	28.1 ± 3.5	22.1 ± 2.1	29.6 ± 4.2	23.3 ± 2.2	32.2 ± 3.0
	P= 0.571		P= 0.102		P= 0.034*
Duration/Amplitude (s/%)	0.09 ± 0.02	0.14 ± 0.02	0.15 ± 0.03	0.13 ± 0.03	0.15 ± 0.06	0.12 ± 0.04
	P= 0.068		P= 0.734		P= 0.684
Area under the curve (%/s2)	7373.1 ± 1743.2	7293.1 ± 1754.3	3166.2 ± 720.8	5022.8 ± 1898.3	4148.5 ± 791.5	9349.9 ± 1809.5
	P= 0.976		P= 0.269		P= 0.027*
Half-width (s)	14.4 ± 2.0	15.3 ± 2.1	10.4 ± 1.4	13.0 ± 2.2	10.3 ± 0.9	16.5 ± 1.6
	P= 0.751		P= 0.336		P= 0.003*
Delay (s)	1.5 ± 0.3	1.6 ± 0.3	3.1 ± 0.9	2.7 ± 0.8	6.9 ± 1.9	4.3 ± 0.8
	P= 0.837		P= 0.783		P= 0.175
Time-to-peak (s)	4.8 ± 0.9	5.9 ± 0.7	6.3 ± 0.8	7.7 ± 1.7	4.6 ± 0.5	6.1 ± 1.2
	P= 0.358		P= 0.431		P= 0.295
Rise slope (10–90%) (%/s)	227.5 ± 57.3	159.6 ± 52.9	96.7 ± 37.5	47.6 ± 15.0	179.1 ± 55.5	215.5 ± 51.5
	P= 0..397		P= 0.537		P= 0.660
Rise time (10–90%) (s)	3.6 ± 1.3	2.7 ± 2.2	4.8 ± 0.9	7.1 ± 1.8	3.1 ± 0.9	5.4 ± 1.9
	P= 0.763		P= 0.235		P= 0.403
Decay slope (10–90%) (%/s)	−36.3 ± 6.5	−27.8 ± 5.3	−27.8 ± 5.7	−17.6 ± 3.3	−46.7 ± 15.8	−32.1 ± 5.4
	P= 0.331		P= 0.286		P= 0.330
Decay time (10–90%) (s)	15.2 ± 2.1	18.2 ± 3.1	10.3 ± 1.1	13.4 ± 1.6	11.2 ± 1.5	15.5 ± 1.7
	P= 0.504		P= 0.127		P= 0.085

Mean values and standard error for different PSC calcium response parameters measured in control and chronically stimulated NMJs. *Significant P values.