Table 2.
Dexamethasone drug levels in ocular tissues from 0.1% mixed micellar formulation
| Average back-calculated calibration curve standards for DEX (n=7) | |||||
|---|---|---|---|---|---|
| Name | Nominal conc.(ng/mL) |
Mean conc.(ng/mL) |
SD (±) |
% CV | % Mean accuracy |
| CCS-1 | 617.60 | 593.15 | 9.69 | 1.6 | 96.0 |
| CCS-2 | 494.08 | 460.95 | 20.38 | 4.4 | 93.3 |
| CCS-3 | 395.26 | 402.59 | 25.26 | 6.3 | 101.9 |
| CCS-4 | 296.45 | 285.68 | 5.64 | 2.0 | 96.3 |
| CCS-5 | 192.69 | 181.27 | 7.85 | 3.3 | 94.1 |
| CCS-6 | 105.98 | 103.51 | 5.22 | 5.0 | 97.7 |
| CCS-7 | 31.79 | 34.41 | 5.09 | 14.8 | 108.2 |
| CCS-8 | 11.13 | 11.07 | 0.65 | 5.9 | 100.6 |
| CCS-9 | 2.70 | 2.71 | 0.30 | 11.2 | 100.4 |
| DEX drug levels in ocular tissues from 0.1% mixed micellar formulation after topical administration | ||
|---|---|---|
| Name of rabbit ocular tissue |
Mean amount of DEX conc. ng/ gm tissue or gm protein |
SD(±) |
| Retina Choroid | 49 | 23.11 |
| Aqueous Humor | 344 | 116.69 |
| Iris Ciliary Muscle | 530* | 309.08 |
| Sclera | 104 | 67.09 |
| Cornea | 1051* | 446.84 |
| Lens | BLOQ | |
| Vitreous Humor | BLOQ | |
BLOQ : Below limit of quantification; LOQ : Limit of quantification ( 2.70 ng/mL)
*
Note: Applied dilution factors for cornea (four times) and iris ciliary muscle (two times) samples