Immunostaining for VEGFR1 (A–B), VEGFR2 (C–D) and VEGFR3 (E–F) in nontransgenic (A, C, E) and G93A-SOD1 (B, D, F) mouse spinal cord sections. Arrows point to VEGFR expression in MNs in the ventral horn. (G) PCR on RNA isolated from G93A-SOD1 and nontransgenic control mouse spinal cords for VEGF family ligands and VEGFR RNA. Densitometry followed by one-way ANOVA and Tukey’s multiple comparison test demonstrates significant decreased expression of VEGF and VEGFR2 RNA in G93A-SOD1 spinal cords compared to nontransgenic control spinal cords (* P < 0.01). Results are normalized to actin. Scale bar 50 µm.