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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Dev Neurobiol. 2009 Nov;69(13):871–884. doi: 10.1002/dneu.20747

Figure 2. VEGFR expression and activation by VEGF in cultured primary MNs.

Figure 2

Immunostaining of primary MNs for VEGFR2 (A) and the MN marker ChAT (B) demonstrates expression of VEGFR2 in MNs (C). Treatment with VEGF (250 ng/ml, 30 min) promotes activation of VEGFR2 as indicated by phosphorylation of Y951 (E) and Y1175 (G) throughout the MN cell body and axon (ChAT positive; arrows) upon VEGF stimulation. Phosphorylation is not seen in the absence of VEGF treatment (D, F). Scale bar 50 µm.