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. Author manuscript; available in PMC: 2011 Mar 16.

Published in final edited form as: Biochemistry. 2010 Mar 16;49(10):2097–2109. doi: 10.1021/bi901977k

NS3h translocation kinetics monitored by stopped-flow experiments with fluorescein labeled ssDNA. NS3h (100 nM) was pre-incubated with 200 nM ssDNA in assay buffer and translocation was initiated by the addition of ATP (5 mM), MgCl₂ (10 mM) and heparin (4 mg/ml). (A) Fluorescence time courses obtained with 5’-(dT)₄₀–F () and 5’-(dT)₆₀-F (). (B) Fluorescence time courses obtained with 5’-F-(dT)₄₀ () and 5’-F-(dT)₆₀ ().

Inline graphic — NS3h translocation kinetics monitored by stopped-flow experiments with fluorescein labeled ssDNA. NS3h (100 nM) was pre-incubated with 200 nM ssDNA in assay buffer and translocation was initiated by the addition of ATP (5 mM), MgCl₂ (10 mM) and heparin (4 mg/ml). (A) Fluorescence time courses obtained with 5’-(dT)₄₀–F () and 5’-(dT)₆₀-F (). (B) Fluorescence time courses obtained with 5’-F-(dT)₄₀ () and 5’-F-(dT)₆₀ ().