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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Lab Invest. 2009 Dec 21;90(2):180–195. doi: 10.1038/labinvest.2009.135

Figure 4.

Figure 4

Long-term (6 days) explant organ culture of E12 lungs evaluated for branching morphogenesis and cell proliferation. Gross appearance of lung explants maintained in either normoxia (a, c, and e) or hypoxia (b, d, and f). Size of lungs increased more than twofold after 6 days in hypoxia (f) compared with normoxia (e). (g) Morphometric (n = 7) assessment of branching morphogenesis showed a significant and sustained increase in airway branching after 3 days in hypoxia compared with cultures maintained under normoxia. (h) Proliferative index (assessed by BrdU labeling; n = 4) of lung E12 organ cultures exposed to 6 days of hypoxia is significantly increased compared with normoxia (*P < 0.05).