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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Lab Invest. 2009 Dec 21;90(2):180–195. doi: 10.1038/labinvest.2009.135

Figure 8.

Figure 8

Analysis for expression of Mash-1 and Hes-1 in lung organ cultures. Western blot (a) and RT-PCR (b) were performed on E12 and E16 lungs in organ culture under normoxia and hypoxia. E12 lungs cultured under normoxia express Mash-1, whereas E12 lungs cultured under hypoxia show almost complete loss of Mash-1 expression. However, when these hypoxia-cultured lung explants were returned to normoxia for 24 h, there was a significant upregulation in Mash-1 expression (recovery). In contrast, E16 lungs did not exhibit any loss of Mash-1 expression under hypoxia. In comparison, Hes-1 expression was maintained under all conditions and gestational ages. Densitometric analysis of western blots shown indicates large differences for Mash-1 and some variance for Hes-1, all relative to positive controls for Mash-1 (brain) and Hes-1 (liver). Loading controls are tubulin for western blots and actin for RT-PCR.