Figure 2.

Progress curves for the inhibition of human neutrophil elastase (HNE) by inhibitor 16. Absorbance was monitored at 410 nm for reaction solutions containing 10 nM HNE, 105 µM MeOSuc-AAPV p-nitroanilide, and the inhibitor at the indicated inhibitor to enzyme ratios in 0.1 M HEPES buffer containing 0.5 M NaCl, pH 7.25, and 2.5% DMSO. The temperature was maintained at 25°C, and reactions were initiated by the addition of enzyme.