Table 2.
Other Enterobacteriaceae used in this study and the results of 4-methyl-β-D-glucuronide (MUG) overlay for the detection of β-glucuronidase-producing bacteria, most probable number (MPN) assay in EC tubes containing MUG, and colony overlay procedure for peptidases (COPP) overlay for lysyl aminopeptidase activity.
| Pathogen | ID code | Source1 | No. MUG+ in triplicate overlays2 | MPN-MUG tubes3 | COPP overlay2 | ||
|---|---|---|---|---|---|---|---|
| Growth | Gas | MUG fluorescence | |||||
| Citrobacter braakii | 43162 | ATCC | 0 | − | − | − | − |
| Citrobacter freundii | 8090 | ATCC | 0 | − | − | − | − |
| Citrobacter freundii | 33128 | USDA | 0 | − | − | − | − |
| Klebsiella pneumonia | 13883 | ATCC | 0 | − | − | − | − |
| Pseudomonas aeruginosa | 7700 | ATCC | 0 | − | − | − | – |
| Pseudomonas fluorescens | 43892 | ATCC | 0 | − | − | − | − |
| Salmonella Dublin | 15480 | ATCC | 0 | ± | − | − | − |
| Salmonella Enteritidis | SAL 196 H 3502 | USDA | 0 | ± | − | − | − |
| Salmonella Montevideo | SAL 2#61 FSIS 051 | USDA | 3 | ± | − | + | − |
| Salmonella Newport | SAL1#86 H 1073 | USDA | 0 | ± | − | − | − |
| Salmonella Typhimurium | G 7601 WI U 302 | USDA | 0 | ± | − | − | − |
| Salmonella Typhimurium | H 3380 CA DT 104 | USDA | 0 | ± | − | − | − |
| Salmonella Typhimurium | SAL 2#46 FSIS 026 | USDA | 0 | ± | − | − | − |
| Serratia liquefaciens | 35551 | ATCC | 0 | − | − | − | − |
| Shigella boydii | 9207 | ATCC | 2 | − | − | − | − |
| Shigella boydii | BS512 | USUHS | 3 | ± | − | + | + |
| Shigella dysenteriae | BS506 | USUHS | 0 | − | − | − | + |
| Shigella flexneri | 2457T | USUHS | 0 | − | − | − | − |
| Shigella flexneri | 12022 | ATCC | 0 | − | − | − | − |
| Shigella sonnei | BS514 | USUHS | 3 | ± | − | + | − |
| Shigella sonnei | 25931 | ATCC | 3 | ± | − | + | − |
| Yersinia enterocolitica | O:8, 12#08 | USDA | 0 | − | − | − | + |
1Abberviaitons: ATCC: American Type Culture Collection, Manassas, Virginia; USDA: United States Department of Agriculture, Agricultural Research Service, Wyndmoor, Pennsylvania; USUHS: Uniformed Services University of the Health Sciences, Bethesda, Maryland.
2MUG and COPP overlays were performed at 37°C.
3MPN-MUG assays were incubated at 44.5°C.