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Photomicrographs of TUNEL, HE, and merged (TUNEL+HE) staining of the hypothalamus of an NC mouse (a), a SOH mouse (b), a CVH mouse (c), and a CTH mouse (d). Apoptosis (green fluorescence; TUNEL staining) broadly colocalized with HE staining (purple color) of neurons to yield the blue color. Apoptosis is associated with pyknosis of the nucleus (b and d) ×400.
