Fig. 2.

A). Representative image of the medial tibial plateaux of MNX animal showing loss of proteoglycan and cartilage fibrillation (large arrows) vascular channels arising from the subchondral bone are evident at the osteochondral junction (small arrows). B). Medial tibial plateu from an MNX animal treated with 5.0 mg/kg/day M503092. The cartilage surface shows no fibrillation but there is some proteoglycan loss as evidence by diminished toluidine blue staining. Fewer vascular channels (small arrow) are seen in treated animals. C). SHAM-operated animal showing intact cartilage and no vascular channels in this section. Sections were stained with toluidene blue and imaged with a small diameter substage diaphragm to demonstrate blood vessels as refractile structures within channels. Scale bars 100 μm.