Skip to main content
. 2010 Jan 4;38(7):2201–2216. doi: 10.1093/nar/gkp1203

Figure 4.

Figure 4.

Knockdown of PRMT6 expression disrupts oestrogen signalling. (A) Western blot showing PRMT6, CARM1 and β-tubulin expression in MCF-7 cells following transfection with control siRNA (Ctrl-siRNA) or siRNA targeting PRMT6 (P6-siRNA-1) and treatment with or without 10−9 M E2 for 12 h as indicated. (B) Graphical representation of PRMT6 expression levels of western blot shown in (A). (C) Q-RT-PCR analysis of PRMT6 RNA levels in MCF-7 cells following transfection by control siRNA or siRNA targeting PRMT6 and treatment with or without 10−9 M E2 for 12 h as indicated. (D) CARM1 expression as detected in (C). (E) PRMT1 expression as detected in (C). (F) GREB1 expression levels following PRMT6 knockdown. RNA was analysed by Q-RT-PCR for expression of GREB1 as in (C). (G) PR expression levels following PRMT6 knockdown. PR levels were examined as in (C). Each data point represents the mean and SD of results from four transfected cultures. Results shown are from a single experiment, which is representative of two independent experiments. *P < 0.005; NS, no significant change compared to treatment with control siRNA.