Figure 5.

Knockdown of PRMT6 and CARM1 expression inhibits oestrogen-stimulated proliferation of breast cancer cells. (A) MCF-7 cells were transfected with control siRNA (Ctrl-siRNA) or siRNA targeting PRMT6 (P6-siRNA-1), CARM1 (C1-siRNA-1) or both PRMT6 and CARM1 (P6-siRNA-1 and C1-siRNA-1). Following treatment with 10⁻⁹ M E2, cell proliferation was determined by ³H-thymidine incorporation. (B) MCF-7 cells were transfected as in (A) and cell proliferation was determined by ³H-thymidine incorporation in the absence of 10⁻⁹ M E2. Each data point represents the mean and SD of results from eight individual cultures. Results are shown from a single experiment, which is representative of two independent experiments. *P < 0.001.