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. 2010 Jan 8;38(7):2302–2313. doi: 10.1093/nar/gkp1222

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Genetic dependency of Rad55–Ser378 phosphorylation in response to a single double strand break in G1-arrested cells. Cultures of wild-type (WDHY2172) and mutant strains (WDHY2534 ddc2-Δ, WDHY2523 rad24-Δ, WDHY2511 rad50-Δ, WDHY2452 rad9-Δ, WDHY2449 mrc1-Δ) were left untreated (lanes 1), HO-endonuclease was induced (lanes 2) or the cells were treated with 0.1% MMS for 2 h (lanes 3) during G1-arrest. Rad55 was immunoprecipitated from equal amounts of cell extracts and analyzed by immunoblotting using polyclonal anti-Rad55 antibodies (left panels, panels 1–3). Rad53 was detected by immunoblotting in total cell extracts of the corresponding samples (right panels, lanes 4–6).