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. 2010 Jan 13;38(7):2498–2511. doi: 10.1093/nar/gkp1225

Table 1.

Tm values and thermodynamic parameters for the duplexes (buffer conditions 10 mM sodium cacodylate, 300 mM NaCl, 0.1 mM EDTA, pH 7.2)

Name Tm (°C)a ΔH (van’t Hoff) (kcal/mol)a −ΔH (1/Tm versus. ln(c)) (kcal/mol)b −ΔS (van’t Hoff) (cal/mol/K)a −ΔG300 (kcal/ mol)c
DR 33.7 ± 0.2 76.7 ± 2.4 74.1 ± 8.0 222 ± 8 7.5
AR 32.4 ± 0.3 86.0 ± 1.8 80.9 ± 10 254 ± 6 4.7
FR 51.2 ± 0.5 88.7 ± 5.9 89.2 ± 5.8 246 ± 18 15.4
DD 37.1 ± 0.3 75.7 ± 1.8 72.4 ± 5.2 216 ± 6 7.6
RR 46.9 ± 0.2 86.8 ± 3.6 93 ± 17 244 ± 11 19.8

aStandard deviations are given.

bErrors are estimated from the 1/Tm versus ln(c) plot.

cCalculated using (1/Tm versus ln(c)) values for ΔH and van’t Hoff values for ΔS.