Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jan 12;38(7):2378–2386. doi: 10.1093/nar/gkp1227

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Molecular and genetic design of taRNA. (A) Schematic diagram showing the function of the taRNA. (B) The most stable structure of the taRNA as predicted by mfold. Each structural and functional unit is indicated by a circle or a rectangle, with the name of the element next to it. The three-way junction connecting the two MS2 coat protein ligands was derived from the 5S rRNA of H. marismortui (47). The other three-way junction was a stable 5S rRNA variant named ‘System F’ (48). (C) Schematic diagram depicting the EcoRI segment in the pDB series of plasmids before and after subcloning, and the RNA transcribed from the synthetic gene before and after further processing by hammerhead ribozyme cleavage. The DNA and RNA segments are aligned to show their relationship. The two ribozymes were taken from peach latent mosaic viroid (PLMVd) and tobacco ringspot virus satellite RNA (sTRSV), respectively (31). The cleavage sites of the ribozymes are indicated by arrowheads.