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. 2010 Jan 12;38(7):2378–2386. doi: 10.1093/nar/gkp1227

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Activity of the taRNA as measured by β-galactosidase activity. (A) Qualitative filter lift assay of permeabilized cells from a ura-, his- plate using X-gal. (B) Quantitative colorimetric assay of cell lysate using ONPG. The average activity of each strain is shown normalized relative to the Gal4 three-hybrid positive control (IRE-MS2 + AD-IRP) (29). Error bars show standard deviations. (C) Optical array analysis of living single cells using C₁₂FDG. A total of 114 cells were measured one and two hours after adding the substrate. The inset shows a section of the array.