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. 2010 Jan 11;38(7):2256–2267. doi: 10.1093/nar/gkp1229

Figure 1.

Figure 1.

HuR is necessary for RNPC1 to regulate p21 expression. (A) The level of p21, HuR, RNPC1a and actin was measured in RKO cells transfected with scrambled siRNA (SCR) or siRNA against HuR for 24 h, followed with or without induction of RNPC1a for 48 h. (B) The level of p21, RNPC1 and HuR transcripts was measured by qRT–PCR with total RNA purified from RKO cells treated as in (A) above. The level of GAPDH transcript was measured as an internal control for normalization. *P < 0.05 (Students’ t-test). (C) The level of p21, HuR, Myc-HuR, RNPC1a and actin was measured in RKO cells transfected with an empty vector or a vector expressing Myc-tagged HuR for 24 h, followed with or without induction of RNPC1a for 48 h.