Fig. 4.
Adrenergic receptor expression in fetal sheep islets. Adrenergic receptors (ADR) were cloned from sheep tissues, and their expression was determined for fetal sheep islets isolated from control or IUGR islets at 135 dGA. PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide. Lanes for each gel include a negative control (no cDNA; −) and positive control (adipose; +) alongside 2 representative samples from islet mRNA extracted from control or IUGR islets. Quantitative PCR was performed (n = 6 controls and n = 7 IUGRs). ΔCT ± SE are presented for control and IUGR islets. Fold change (fold Δ) in IUGR islets from control islets was calculated by the 2−ΔΔCT method for all adrenergic receptor isoforms, and results are presented. Statistical analysis was performed on ΔCT values with ribosomal protein S15 as the reference gene (*P < 0.05 between treatment groups).